Induction of the metastatic phenotype by transfection of a benign rat mammary epithelial cell line with the gene for p9Ka, a rat calcium-binding protein, but not with the oncogene EJ-ras-1

摘要: The rat mammary epithelial cell line, Rama 37, yields benign, non-metastasizing adenomatous tumours in syngeneic Wistar-Furth rats. Transfection of this line with a drug resistance plasmid containing both the gene for to Geneticin (neo) and p9Ka (pSV2neo-p9Ka), calcium-binding protein, or similar neo oncogene EJ-ras-1 (pSV2neo-ras) drug-resistant transformants that express high levels mRNAs proteins. These transfected cells all produce when injected at subcutaneous sites shorter median latent period than produced by parental untransfected 37 hosts. Cells pSV2neo-p9Ka yield higher incidence cells, many which metastasize lungs and/or lymph nodes However, pSV2-neo-ras pSV2neo alone fail metastasize. Immunofluorescent studies suggest an association cytoskeleton, as depicted F-actin staining reagent phalloidin. It is suggested transfection copies protein can enhance tumorigenic potential induce metastatic phenotype model, whereas control DNA fails phenotype, although transfectants, like those p9Ka, possess increased growth properties vivo.