Characterization of a Canine Glioma Cell Line As Related to Established Experimental Brain Tumor Models

摘要: A large animal tumor model for anaplastic glioma has been recently developed using immunotolerant allogeneic Beagle dogs and an established canine cell line, J3T. This offers advantages in terms of morphology similarity to human glioma. The present study was aimed at evaluating the biological characteristics J3T line as related experimental gene therapy studies. Furthermore, development brain tumors a xenogeneic immunodeficient SCID mouse investigated. It demonstrated that cultured cells can be efficiently infected by adenovirus (AV), herpes-simplex type I (HSV), or retrovirus (RV) vectors, well non-virus vectors such cationic liposome/DNA complexes. Thus, infectability transfectability, seem closer than 9L rodent gliosarcoma. Cytotoxicity selection antibiotics G418, puromycin, hygromycin on essentially resemble cytotoxicity seen with other lines, example, 9L, U87, U343. RV-mediated HSV-TK/GCV comparable LD50 TK-expressing control (non-expressing) treated Ganciclovir. Further, it proven are tumorigenic may grow heterotopically orthotopically host, mouse, although growth pattern these differ from invasive phenotype dog.