Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.

摘要: Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and 27) transformed with Agrobacterium rhizogenes A4M70GUS their corresponding regenerated shoot culture (HRRS) were compared for differences in growth, production phenolic compounds, antioxidant antimicrobial activities. Transgenic selected on the basis morphological evaluation, genetic molecular analyses. The clone 2 had highest biomass accumulation, while 27 showed regeneration potential. total phenolics flavan-3-ols enhanced all tested transgenic cultures, flavonoids hypericins augmented HRRS to non-transformed shoots. produced substantial amounts chlorogenic acid 3-p-coumaroylquinic acid. Regarding flavonoids, they significant contents luteolin hexoside (HRRS 2), quercitrin quercetin 15) isoquercetin 27), accumulated 4-O-methylkaempferol-O-hexoside 6-C-glucoside, respectively. was promising catechin procyanidin derivatives together its exhibited a high potential hyperforin adhyperforin production. All identified naphtodianthrones confirmed clones. Among xanthones, mangiferin found as major compound HRRS, trihydroxy-1-metoxy-C-prenyl xanthone dominant Antimicrobial activity cultures revealed that strongly inhibited growth Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa Escherichia coli. Altogether, H. could be proposed experimental systems compounds antibacterial properties.