Modeling of flow-induced shear stress applied on 3D cellular scaffolds: Implications for vascular tissue engineering.

摘要: Novel tissue-culture bioreactors employ flow-induced shear stress as a means of mechanical stimulation cells. We developed computational fluid dynamics model the complex three-dimensional (3D) microstructure porous scaffold incubated in direct perfusion bioreactor. Our was designed to predict high shear-stress values within physiological range those naturally sensed by vascular cells (1–10 dyne/cm2), and will thereby provide suitable conditions for tissue-engineering experiments. The also accounts cellular growth, which an added cell layer grown on all walls. Five variants were designed, with geometric differences corresponding cell-layer thicknesses 0, 50, 75, 100, 125 µm. Four inlet velocities (0.5, 1, 1.5, 2 cm/s) applied each model. Wall distribution overall pressure drop calculations then used characterize relation between flow rate, stress, thickness, drop. simulations showed that growth 3D scaffolds exposes elevated considerably increasing average correlation inflow velocity. results in-depth analysis microdynamic environment cultured environments, thus advanced control over tissue development vitro. Biotechnol. Bioeng. 2010; 105: 645–654. © 2009 Wiley Periodicals, Inc.