Enantioselective Phytotoxicity of Dichlorprop to Arabidopsis thaliana: The Effect of Cytochrome P450 Enzymes and the Role of Fe

摘要: The ecotoxicology effects of chiral herbicides have long been recognized and drawn increasing attention. toxic mechanisms in plants are involved production reactive oxygen species (ROS) cause damage to target enzymes, but the relationship between these two factors enantioselectivity has rarely investigated. Furthermore, even though cytochromes P450 enzymes (CYP450s) related phytotoxicity herbicides, their roles yet be explored. To solve this puzzle, CYP450s suicide inhibitor 1-aminobenzotriazole (ABT) was added an exposure system made from dichlorprop (DCPP) enantiomers model plant Arabidopsis thaliana. results indicated that different phytotoxicities DCPP by causing oxidative stress acetyl-CoA carboxylase (ACCase) were observed presence absence ABT. addition ABT decreased toxicity (R)-DCPP not significantly affected (S)-DCPP, resulting smaller differences enantiomers. profound also Fe uptake distribution, exhibiting distribution patterns A. thaliana leaves exposed ABT, which helped bridge ROS enzyme ACCase through function CYP450s. These offer opportunity for a more-comprehensive understanding herbicide action mechanism provide basic evidence risk assessments environment.