Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells
作者： Xudong SUN , Huimin YU , Zhongyao SHEN
DOI: 10.1016/S1004-9541(08)60282-7
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摘要: Abstract Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile. The deactivation kinetics of NHases in free resting cells Rhodococcus sp. was presented based on a bi-steady state assumption. Effects hydration temperature, product concentration and substrate NHase were investigated experimentally correlated with first order kinetics. results showed that the temperature major factors governing under substrate-feeding conditions. When higher than 250 g·L−1, became serious assumption not applicable. controlled at relatively level such as 28°C, total rate constant about 2.8-fold 20°C.
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