A new enzymatic method of acrylamide production.

摘要: To produce acrylamide from acrylonitrile by use of a new enzyme, nitrile hydratase, number nitrile-utilizing microorganisms were screened for the enzyme activity an intact cell system. An isobutyronitrile-utilizing bacterium, strain B23, showed best productivity among 186 strains tested. The was identified taxonomically as Pseudomonas chlor or aphis. culture and reaction conditions production studied strain. Under optimum conditions, 400 grams/liter produced in 7.5 hr. yield nearly 100% with trace amount acrylic acid. cell-free extract strong hydratase toward extremely low amidase acrylamide.