Hydrogen peroxide regulates elicitor PB90-induced cell death and defense in non-heading Chinese cabbage

摘要: Abstract PB90, a protein elicitor secreted by Phytophthora boehmeriae , can induce hypersensitive response and systemic acquired resistance in tobacco. In this study, we first developed novel plant-elicitor interaction system between PB90 non-heading Chinese cabbage, then reported PB90-induced programmed cell death defense regulated hydrogen peroxide (H 2 O ) the plant leaves. The shared common morphological characteristics of apoptosis with mammalian death, including rapid chromatin condensation cytoplasmic shrinkage, suggesting that is occurrence. At same time, treatment strongly activated enhanced phenylalanine ammonia-lyase (PAL) activity. also resulted PR-1 Chitinase expression, as evaluated reverse transcriptase–polymerase chain reaction, concomitant plants to infection Colletotrichum higginsianum Erwinia carotovora var. . Moreover, oxidative burst, result H accumulation over approximately 3 h, preceded PB90-triggered 12 h post-infiltration PB90. Infiltration antioxidants (DTT, glutathione, ascorbic acid) DPI, an inhibitor NADPH oxidase, into leaves 30 min before significantly suppressed expression We therefore, provided evidence burst triggered cabbage.