Thalidomide and the immune system. 3. Simultaneous up- and down-regulation of different integrin receptors on human white blood cells.

摘要: Abstract Time-dependent changes in the surface receptor expression of various maturational and integrin receptors on peripheral blood cells were studied two healthy human volunteers following oral applications thalidomide (Thd). In each measurement density was quantified by prior calibration flow cytometer with latex beads bearing a determined number fluorescence molecules. The effects observed course Thd-treatment practically identical or at least very similar both during four different trials, accord previous results obtained large-scale studies (68 treated animals) non-human primates. It should be stressed that no clear-cut percentage absolute numbers primary lymphocyte subsets such as CD3, CD4 CD20. After first doses 7 mg Thd/kg body wt CD18 (the common β-chain β2-integrins) marker already decreased longer detectable granulocytes, monocytes lymphocytes. This effect persisted throughout treatment period slowly subsided after discontinuation treatment. With few days lag phase, CD54 (ICAM-1) granulocytes increased many previously not this newly acquired markers. On however, lost cells. Within fraction loss could noted but CD8 cells, where an increase observed. Other markers, alpha chains β1 integrins CD49b (VLAα2) CD49d (VLAα4) showed contrasting reactions to Thd-treatment. Whereas pronounced only high epitope left end complete dosing schedule, observable epitope. A distinct reduction also noticeable L-selectin (Leu8) positive lymphocytes, majority described adhension seen CD45R0 functional is strongly down-regulated pathway CD45RA maturation apparently altered These multiple we may explain large variety therapeutic experienced Thd.