Microarray analysis of PBMC after Plasmodium falciparum infection: Molecular insights into disease pathogenesis.

摘要: Abstract Objective To find out host gene expression profiles after malarial infection. Methods Further time-course microarray analysis of peripheral blood mononuclear cells focusing on malaria pathogenesis was performed. Results Up-regulation coagulation-related genes, heat shock proteins, glycolytic enzymes, glucose transporters, and vacuolar H + -ATPases found in acute febrile malaria. In early malaria, prior to detectable parasitemia, CD36 ICAM1 were up-regulated. During there is correlation between IL-1β proteins. CD163, a hemoglobin scavenger receptor, up-regulated potentially facilitate free up-take by leukocytes. high MafB negatively correlated with platelet counts. Consistent down-regulation, red cell counts tended increase during Up-regulations leukocyte binding mediators like CD36, ICAM1, thrombospondin, thrombomodulin may contribute the cerebral Similarly, up-regulation transporter hypoglycemia metabolic acidosis frequently observed serious patients. Overall gender effects male female not apparent, except for some hemoglobins significantly down-regulated versus female, which suggesting males are prone malaria-related anemia. Conclusions Leukocyte can explain complication such as fever, acidosis, hypoglycemia, anemia, coagulopathy.