Mono-epoxy-tocotrienol-α enhances wound healing in diabetic mice and stimulates in vitro angiogenesis and cell migration.

摘要: Diabetes mellitus is characterized by hyperglycemia and capillary hypoxia that causes excessive production of free radicals impaired antioxidant defense, resulting in oxidative stress diabetes complications such as wound healing. We have previously shown modified forms tocotrienols possess beneficial effects on the biosynthesis mevalonate pathway lipids including increase mitochondrial CoQ. The aim this study to investigate mono-epoxy-tocotrienol-α vitro vivo healing models well its function. Gene profiling analysis gene expression studies HepG2 cells human dermal fibroblasts were performed microarray qPCR, respectively. In using was studied scratch assay angiogenesis microvascular endothelial tube formation assay. diabetic db/db mouse model. For functions oxygen consumption rate Seahorse XF-24 employed. vitro, significant closure cell migration (p<0.05) both normal high glucose (angiogenesis) (p<0.005) observed. Microarray showed a 20-fold KIF26A 11-fold decrease lanosterol synthase expression. Expression qPCR growth factors VEGFA PDGFB. epoxidated compound induced significantly higher basal reserve capacity HDF cells. Additionally, mice, demonstrated small but enhancement upon local application compared treatment with vehicle alone. Mono-epoxy-tocotrienol-α seems increasing genes involved growth, motility angiogenes