Molecular and biological properties of c-mil transducing retroviruses generated during passage of Rous-associated virus type 1 in chicken neuroretina cells.
作者： A Eychène , C Béchade , M Marx , D Laugier , P Dezélée
DOI: 10.1128/JVI.64.1.231-238.1990
关键词:
摘要: IC1, IC2, and IC3 are novel c-mil transducing retroviruses generated during serial passaging of Rous-associated virus type 1 (RAV-1) in chicken embryo neuroretina cells. They were isolated by their ability to induce proliferation these nondividing IC2 early passages RAV-1 cells, whereas IC1 was after six consecutive supernatants. We sequenced the transduced genes mil-RAV-1 junctions three viruses. The 5' RAV-1-mil junction formed a splicing process between leader sequence exon 8 gene. end resulted from homologous recombination gag mil sequences. Reconstitution experiments showed that cells also led formation gag-mil-containing retrovirus. Therefore, constitution U5-leader-delta c-mil-delta RAV-1-U3 represents steps transduction RAV-1. This further recombined with generate virus. IC viruses serine/threonine kinase domain cellular Hence, amino-terminal truncation is sufficient activate mitogenic property c-mil. Comparison transforming properties gene, expressed as unique protein independent sequences, weakly avian Acquisition sequences not only increased rate replication but enhanced capacity
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