[5] Avian neuroretina cells in oncogene studies

摘要: Publisher Summary This chapter presents the two main reasons to use cells from avian embryonic neuroretina (NR) as an experimental system study effects of oncogenes on regulation cell growth and differentiation. First, NR cultures are exclusively composed neuroectodermal cells: this tissue is not vascularized does contain mesenchymal origin. Second, when these maintained in culture, they rapidly stop dividing express several markers neural The development proceeds through essentially three phases: proliferation precursor cells, lamination strata, differentiation postmitotic cells. discusses that obtained 7-day-old chicken embryos can be monolayers able differentiate into neuronal nonneuronal (glial) stage at which morphologically mature synapses found. It, therefore, appears synaptogenesis primarily depends genetic programs complex interactions, found tridimensional structures, may required for formation synapses. also highlights changing culture conditions influence pattern Nr provide a convenient model vitro studies activation transduction protooncogenes by retroviruses.