Overexpression of the MDM2 gene by childhood acute lymphoblastic leukemia cells expressing the wild-type p53 gene.

摘要: The wild-type (wt) p53 tumor suppressor gene is commonly inactivated in human malignancies, either by mutations or loss of expression. An additional proposed mechanism for inactivation wt-p53 amplification the murine double minute 2 (MDM2) and overexpression MDM2 protein, which binds to eliminates its function. To investigate a potential role pediatric acute lymphoblastic leukemia (ALL), we examined expression p53, as well occurrence possible gene, 19 ALL cell lines one myelogenous (AML) line. Although did not find significant any leukemic lines, detected all 10 that expressed wt-p53. Of without six (including AML line) express four mutant with single point exons 7 8. determine whether primary cells showed similar correlation, analyzed original cryopreserved bone marrow from seven patients whom were established. We obtained results both corresponding lines: was present but lacked These findings suggest an important pathogenesis