Ovarian thecal/interstitial cells produce an epidermal growth factor-like substance
作者： MICHAEL K. SKINNER , DEREK LOBB , JENNIFER H. DORRINGTON
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摘要: An ovarian thecal/interstitial cell preparation was obtained by enzymatic digestion of immature rat ovaries depleted granulosa cells. A component in cell-conditioned medium inhibited specific binding [125I]iodo-epidermal growth factor ([125I]EGF) to its surface receptor, as determined with an EGF radioreceptor assay. Thecal/interstitial secreted proteins also stimulated EGF-dependent line. Granulosa cells isolated from the same did not produce detectable levels EGF-like substance, both RRA and Fractionation size exclusion HPLC resulted a 35K being detected substance when were fractionated reverse phase HPLC. Several biochemical properties examined different those authentic mouse EGF. However, biological activities similar their ability promote In addition production produced inhibitory activity. This product stimulate growth. receptors are present on cells, is known influence differentiation these Observations indicate that may act paracrine regulate differentiation. addition, activity have role regulating follicular
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sci-hub.se 参考文章(33)
M Rao, Hormonal regulation of ovarian cellular proliferation Cell. ,vol. 14, pp. 71- 78 ,(1978) , 10.1016/0092-8674(78)90302-1
Stanley Cohen, Isolation of a Mouse Submaxillary Gland Protein Accelerating Incisor Eruption and Eyelid Opening in the New-born Animal Journal of Biological Chemistry. ,vol. 237, pp. 1555- 1562 ,(1962) , 10.1016/S0021-9258(19)83739-0
P B Jones, T H Welsh, A J Hsueh, Regulation of ovarian progestin production by epidermal growth factor in cultured rat granulosa cells. Journal of Biological Chemistry. ,vol. 257, pp. 11268- 11273 ,(1982) , 10.1016/S0021-9258(18)33752-9
John M. Taylor, William M. Mitchell, Stanley Cohen, Epidermal Growth Factor Journal of Biological Chemistry. ,vol. 247, pp. 5928- 5934 ,(1972) , 10.1016/S0021-9258(19)44847-3
P Feng, K J Catt, M Knecht, Transforming growth factor beta regulates the inhibitory actions of epidermal growth factor during granulosa cell differentiation. Journal of Biological Chemistry. ,vol. 261, pp. 14167- 14170 ,(1986) , 10.1016/S0021-9258(18)66998-4
Edward B. Leof, Harold L. Moses, Gary D. Shipley, Anton Scott Goustin, Growth Factors and Cancer Cancer Research. ,vol. 46, pp. 1015- 1029 ,(1986)
Stanley Cohen, Epidermal growth factor Bioscience Reports. ,vol. 6, pp. 1017- 1028 ,(1986) , 10.1007/BF01141022
Alane Gray, Thomas J. Dull, Axel Ullrich, Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor Nature. ,vol. 303, pp. 722- 725 ,(1983) , 10.1038/303722A0
Gregory F. Erickson, Elizabeth Case, Epidermal growth factor antagonizes ovarian theca-interstitial cytodifferentiation Molecular and Cellular Endocrinology. ,vol. 31, pp. 71- 76 ,(1983) , 10.1016/0303-7207(83)90031-X
R. B. DICKSON, K. K. HUFF, E. M. SPENCER, M. E. LIPPMAN, Induction of Epidermal Growth Factor-Related Polypeptides by 17β-Estradiol in MCF-7 Human Breast Cancer Cells Endocrinology. ,vol. 118, pp. 138- 142 ,(1986) , 10.1210/ENDO-118-1-138