The crosstalk between osteoclasts and osteoblasts is dependent upon the composition and structure of biphasic calcium phosphates

摘要: Biphasic calcium phosphates (BCPs), consisting of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), exhibit good biocompatibility osteoconductivity, maintaining a balance between resorption the biomaterial formation new bone. We tested whether chemical composition and/or microstructure BCPs affect osteoclasts (OCs) differentiation their ability to crosstalk with osteoblasts (OBs). To this aim, OCs were cultured on HA content 5, 20 or 60% activity assessed. found that OC is partially impaired by increased content, but not presence micropores within BCP scaffolds, as indicated TRAP staining gene profile expression. then investigated biomaterial-induced changes in also OBs regulate OB function. low percentage favored expression positive coupling factors, including sphingosine-kinase 1 (SPHK1) collagen triple helix repeat containing (Cthrc1). In turn, increase these secreted factors promotes All together our studies suggest biomaterials affects only potential locally bone formation.