The regulatory function of dIno80 correlates with its DNA binding activity
作者： S Jain , J Maini , A Narang , S Maiti , V Brahmachari
DOI: 10.1101/519959
关键词:
摘要: The INO80 complex, including the Ino80 protein, forms a highly conserved canonical complex that remodels chromatin in context of multiple cellular functions. Drosophila homologue, dIno80, is involved homeotic gene regulation during development as Pho-dIno80 complex. Previously, we found dIno80 regulates genes by interacting with epigenetic regulators, such polycomb and trithorax, suggesting occurrence non-canonical complexes. Here using spectroscopic methods gel retardation assays, identified set consensus DNA sequences binding domain (DBINO) interacts having differential affinity high specificity. Testing these reporter showed this interaction can positively regulate transcription. These results suggest that, has sequence preference for leading to transcriptional changes.
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