Synthesis of defined peptide-oligonucleotide hybrids containing a nuclear transport signal sequence.

作者: Ramon Eritja , Anna Pons , Mónica Escarcellar , Ernest Giralt , Fernando Albericio

DOI: 10.1016/S0040-4020(01)86448-6

关键词:

摘要: Abstract Oligonucleotide-peptide hybrid molecules containing a nuclear transport signal have been synthesized using two different approaches based on the specific reactivity of thiol group.

参考文章(18)
M.H. Caruthers, A.D. Barone, S.L. Beaucage, D.R. Dodds, E.F. Fisher, L.J. McBride, M. Matteucci, Z. Stabinsky, J.-Y. Tang, Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method. Methods in Enzymology. ,vol. 154, pp. 287- 313 ,(1987) , 10.1016/0076-6879(87)54081-2
T. R. Bürglin, E. M. De Robertis, The nuclear migration signal of Xenopus laevis nucleoplasmin. The EMBO Journal. ,vol. 6, pp. 2617- 2625 ,(1987) , 10.1002/J.1460-2075.1987.TB02552.X
Gerald Zon, Oligonucleotide Analogues as Potential Chemotherapeutic Agents Pharmaceutical Research. ,vol. 5, pp. 539- 549 ,(1988) , 10.1023/A:1015985728434
Louis A. Carpino, Oxidative Reactions of Hydrazines. IV. Elimination of Nitrogen from 1,1-Disubstituted-2-arenesulfonhydrazides1-4 Journal of the American Chemical Society. ,vol. 79, pp. 4427- 4431 ,(1957) , 10.1021/JA01573A050
Fernando Albericio, Nancy Kneib-Cordonier, Sara Biancalana, Lajos Gera, R. Irene Masada, Derek Hudson, George Barany, Preparation and application of the 5-(4-(9-fluorenylmethyloxycarbonyl)aminomethyl-3,5-dimethoxyphenoxy)-valeric acid (PAL) handle for the solid-phase synthesis of C-terminal peptide amides under mild conditions Journal of Organic Chemistry. ,vol. 55, pp. 3730- 3743 ,(1990) , 10.1021/JO00299A011
Virender K. Sarin, Stephen B.H. Kent, James P. Tam, R.B. Merrifield, Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction. Analytical Biochemistry. ,vol. 117, pp. 147- 157 ,(1981) , 10.1016/0003-2697(81)90704-1
JOHANNES MEIENHOFER, MICHINORI WAKI, EDGAR P. HEIMRE, THEODORE J. LAMBROS, RAYMOND C. MAKOFSKE, CHI-DEU CHANG, Solid phase synthesis without repetitive acidolysis. Preparation of leucyl-alanyl-glycyl-valine using 9-fluorenylmethyloxycarbonylamino acids. International Journal of Peptide and Protein Research. ,vol. 13, pp. 35- 42 ,(2009) , 10.1111/J.1399-3011.1979.TB01847.X
Jim Haralambidis, Lucy Duncan, Geoffrey W. Tregear, The solid phase synthesis of oligonucleotides containing a 3′-peptide moiety Tetrahedron Letters. ,vol. 28, pp. 5199- 5202 ,(1987) , 10.1016/S0040-4039(00)95628-4
Jim Haralambidis, Lucy Duncan, Karin Angus, Geoffrey W. Tregear, The synthesis of polyamide-oligonucleotide conjugate molecules Nucleic Acids Research. ,vol. 18, pp. 493- 499 ,(1990) , 10.1093/NAR/18.3.493