The presence of circulating tumor cells (CTCs) correlates with lymph node metastasis in nonresectable squamous cell carcinoma of the head and neck region (SCCHN)

摘要: Background: The mechanisms regulating tumor cell dissemination in locally advanced squamous carcinoma of the head and neck region (SCCHN) are largely unresolved. We assessed frequency circulating cells (CTCs), their association with clinicopathologic parameters kinetics during radiochemotherapy. Patients methods: Peripheral blood samples from 42 patients SCCHN were included. CTCs detected using flow cytometric analysis CD452epithelial adhesion molecule+cytokeratin+ results validated by nested RT-PCR epidermal growth factor receptor transcripts. between presence T stage, volume, N stage human papillomavirus status was evaluated. influence radiochemotherapy on CTC numbers determined. Results: 18 (43%), a mean 6 standard deviation 1.7 0.9 per 3.75 ml blood. observed no significant correlation or volume. However, nodal N2b higher associated CTCs. Though concurrent reduced frequency, persisted treatment 20% cases. Conclusions: Detection correlates regional metastasis inoperable SCCHN. Further follow-up is needed to evaluate prognostic significance detection, addition clinical staging lymph nodes, for distant recurrence.