Characterisation of the anti-microbial activity of bovine milk ribonuclease4 and ribonuclease5 (angiogenin)

摘要: Abstract Two members of the ribonucleaseA (RNaseA) family were identified in cows' milk. RNase5, already known to be present, was found at concentrations between 3 and 19 μg mL−1. RNase4, which not previously milk, 1 4 μg mL−1. These proteins purified from Purified RNase5 suppressed growth Candida albicans, particularly its hyphal form, but any other microbes tested. The required suppress higher than those RNase4 had no suppression activity. Both RNases reduced number viable C. albicans cells, with being more potent. This activity abrogated by physiological NaCl. Although both bound cell membrane, their mode action through membrane lysis or permeabilization. Together, these results suggest that bovine milk do have intrinsic anti-microbial