Promoter hypermethylation of the gene encoding heat shock protein B1 in oral squamous carcinoma cells
作者： Hsueh-Chun Wang , Wei-Fan Chiang , Hsin-Hsiu Huang , Shau-Ku Huang , Hung-Che Chiang
DOI: 10.1016/J.OOOO.2012.12.011
关键词:
摘要: Objective The mechanism responsible for the regulation of HSPB1 expression in oral squamous cell carcinoma was explored this study. Study Design and methylation status cells were examined using real-time reverse transcription-PCR, methylation-specific PCR pyrosequencing. Results weakly expressed lines (N = 4) as compared to that normal human keratinocytes. lower associated with promoter hypermethylation gene, could be induced by treating a DNA methyltransferase inhibitor, RG108. Promoter gene also noted primary carcinomas, concomitant reduced levels expression, those paired neighboring tissues. Conclusion Aberrant may explain cancer cells.
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Francesco Carinci, Giuseppina Campisi, Silvia Falaschini, Lorenzo Lo Muzio, Antonio Farina, Gaetano De Rosa, Rosalia Leonardi, Corrado Rubini, Stefania Stalbano, Francesca Ferrari, Prognostic Value of HSP27 in Head and Neck Squamous Cell Carcinoma: a Retrospective Analysis of 57 Tumours Anticancer Research. ,vol. 26, pp. 1343- 1349 ,(2006)
Michael R. Rountree, Kurtis E. Bachman, Stephen B. Baylin, DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci Nature Genetics. ,vol. 25, pp. 269- 277 ,(2000) , 10.1038/77023
Peter A. Jones, Stephen B. Baylin, The fundamental role of epigenetic events in cancer Nature Reviews Genetics. ,vol. 3, pp. 415- 428 ,(2002) , 10.1038/NRG816
François Fuks, Wendy A. Burgers, Alexander Brehm, Luke Hughes-Davies, Tony Kouzarides, DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nature Genetics. ,vol. 24, pp. 88- 91 ,(2000) , 10.1038/71750
F Trautinger, I Kindås-Mügge, Increased expression of the M(r) 27,000 heat shock protein (hsp27) in in vitro differentiated normal human keratinocytes. Cell Growth & Differentiation. ,vol. 5, pp. 777- 781 ,(1994)
Chu Chen, Eduardo Méndez, John Houck, Wenhong Fan, Pawadee Lohavanichbutr, Dave Doody, Bevan Yueh, Neal D. Futran, Melissa Upton, D. Gregory Farwell, Stephen M. Schwartz, Lue Ping Zhao, Gene Expression Profiling Identifies Genes Predictive of Oral Squamous Cell Carcinoma Cancer Epidemiology, Biomarkers & Prevention. ,vol. 17, pp. 2152- 2162 ,(2008) , 10.1158/1055-9965.EPI-07-2893
Anxun Wang, Xiqiang Liu, Shihu Sheng, Hui Ye, Tingsheng Peng, Fei Shi, David L Crowe, Xiaofeng Zhou, Dysregulation of heat shock protein 27 expression in oral tongue squamous cell carcinoma BMC Cancer. ,vol. 9, pp. 167- 167 ,(2009) , 10.1186/1471-2407-9-167
Li-Ping Weng, Chih-Ching Wu, Bao-Lian Hsu, Lang-Ming Chi, Ying Liang, Ching-Ping Tseng, Ling-Ling Hsieh, Jau-Song Yu, Secretome-based identification of Mac-2 binding protein as a potential oral cancer marker involved in cell growth and motility. Journal of Proteome Research. ,vol. 7, pp. 3765- 3775 ,(2008) , 10.1021/PR800042N
Jesus Espada, Hector Peinado, Lidia Lopez-Serra, Fernando Setien, Paula Lopez-Serra, Anna Portela, Jaime Renart, Elisa Carrasco, María Calvo, Angeles Juarranz, Amparo Cano, Manel Esteller, None, Regulation of SNAIL1 and E-cadherin function by DNMT1 in a DNA methylation-independent context Nucleic Acids Research. ,vol. 39, pp. 9194- 9205 ,(2011) , 10.1093/NAR/GKR658
Kurtis E Bachman, Ben Ho Park, Ina Rhee, Harith Rajagopalan, James G Herman, Stephen B Baylin, Kenneth W Kinzler, Bert Vogelstein, Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene. Cancer Cell. ,vol. 3, pp. 89- 95 ,(2003) , 10.1016/S1535-6108(02)00234-9