Regulation of SNAIL1 and E-cadherin function by DNMT1 in a DNA methylation-independent context

摘要: Mammalian DNA methyltransferase 1 (DNMT1) is essential for maintaining methylation patterns after cell division. Disruption of DNMT1 catalytic activity results in whole genome cytosine demethylation CpG dinucleotides, promoting severe dysfunctions somatic cells and during embryonic development. While these observations indicate that DNMT1-dependent required proper function, the possibility has a role independent its matter controversy. Here, we provide evidence can support functions do not require C-terminal domain. We report PCNA DMAP1 domains N-terminal region are sufficient to modulate E-cadherin expression absence noticeable changes gene promoters involved. Changes directly associated with regulation β-catenin-dependent transcription. Present suggests acts on through direct interaction transcriptional repressor SNAIL1.