Identification and purification of resveratrol targeting proteins using immobilized resveratrol affinity chromatography

摘要: The phytochemical resveratrol (trans-3,4',5-trihydroxystilbene) is a naturally occurring polyphenol with plethora of health-beneficial properties, including preventive role in cancer. We surmise that may exert its diverse biological effects by interacting specific target proteins, denoted RTPs. To test this possibility, was immobilized on epoxy-activated agarose forming affinity column (RAC), which used to detect and isolate Distinct RTPs can be resolved RAC fractionation increasing NaCl, followed 1mM ATP, finally, 1-2mM resveratrol. A 22-kDa polypeptide, RTP-22, eluted identified MALDI-TOF MS cloning/expression Escherichia coli, as dihydronicotinamide riboside quinone reductase 2 (NQO2). utility additionally explored extracts derived from different staging prostate cancer cells. NQO2 most abundant CWR22Rv1, model for transition androgen-dependent the hormone-refractory state, but marginally expressed JCA-1 cells representing more advanced stage These results provide evidence existence distinctive mammalian facile approach identify purify