CKβ-11/Macrophage Inflammatory Protein-3β/EBI1-Ligand Chemokine Is an Efficacious Chemoattractant for T and B Cells
作者： Hal E. Broxmeyer , Louis M. Pelus , Kyung Johanson , Chang H. Kim , Edward Applebaum
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摘要: We examined the functional properties of CK beta-11/MIP-3 beta/ELC, a recently reported CC chemokine that specifically binds to receptor, EBI1/BLR2/CCR7. beta/ELC is distantly related other and CXC chemokines in primary amino acid sequence structure. Recombinant human expressed from mammalian cell system showed potent chemotactic activity for T cells B but not granulocytes monocytes. An optimal concentration attracted most input within 3 h, comparable with stromal derived factor 1 (SDF-1), highly efficacious chemokine. equally naive CD45RA+ memory type CD45RO+ cells. also strongly both CD4+ CD8+ cells, attraction was greater. more chemoattractant than MIP-1 alpha, known chemoattractant. induced actin polymerization lymphocytes, chemotaxis completely blocked by pertussis toxin showing its likely EBI1/BLR2/CCR7, coupled G(alpha i) protein. calcium mobilization which could be desensitized SDF-1, suggesting possible cross-regulation their signaling. Human murine splenocytes conservation between mouse. The efficacy chemoattraction tissue expression mRNA suggest may important trafficking thymus, migration secondary lymphoid organs.
jimmunol.org 参考文章(46)
R P M Negus, The chemokines: cytokines that direct leukocyte migration. Journal of the Royal Society of Medicine. ,vol. 89, pp. 312- 314 ,(1996) , 10.1177/014107689608900603
GM Bokoch, Chemoattractant signaling and leukocyte activation Blood. ,vol. 86, pp. 1649- 1660 ,(1995) , 10.1182/BLOOD.V86.5.1649.BLOODJOURNAL8651649
MR Loken, VO Shah, KL Dattilio, CI Civin, Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. ,vol. 70, pp. 1316- 1324 ,(1987) , 10.1182/BLOOD.V70.5.1316.1316
Chang H. Kim, Hal E. Broxmeyer, In Vitro Behavior of Hematopoietic Progenitor Cells Under the Influence of Chemoattractants: Stromal Cell–Derived Factor-1, Steel Factor, and the Bone Marrow Environment Blood. ,vol. 91, pp. 100- 110 ,(1998) , 10.1182/BLOOD.V91.1.100
M Storgaard, N Mukaida, K Thestrup-Pedersen, J Bonde, K Matsushima, B Møller, T Jinquan, C G Larsen, N Grunnet, F T Black, Chemotaxis and IL-8 receptor expression in B cells from normal and HIV-infected subjects. Journal of Immunology. ,vol. 158, pp. 475- 484 ,(1997)
Arieh Katz, Dianqing Wu, Melvin I. Simon, Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C Nature. ,vol. 360, pp. 686- 689 ,(1992) , 10.1038/360686A0
Alan M. Krensky, Thomas J. Schall, Carol Clayberger, Bradley J. Dyer, Mark M. Davis, Jan Jongstra, Jeffrey L Jorgensen, A human T cell-specific molecule is a member of a new gene family. Journal of Immunology. ,vol. 141, pp. 1018- 1025 ,(1988)
K Franz-Bacon, A P Vicari, D L Rossi, T K McClanahan, A Zlotnik, Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta. Journal of Immunology. ,vol. 158, pp. 1033- 1036 ,(1997)
N W Lukacs, R M Strieter, L M Colletti, G B Huffnagle, T J Standiford, S L Kunkel, "The good, the bad, and the ugly." The role of chemokines in models of human disease. Journal of Immunology. ,vol. 156, pp. 3583- 3586 ,(1996)
F. W. Luscinskas, M. A. Gimbrone, J. B. Baker, Han Ding, J. M. Kiely, C. A. Hebert, M. S. Obin, In vitro inhibitory effect of IL-8 and other chemoattractants on neutrophil-endothelial adhesive interactions. Journal of Immunology. ,vol. 149, pp. 2163- 2171 ,(1992)