Determination of chloroquine and desethylchloroquine in plasma and blood cells of Plasmodium vivax malaria cases using liquid chromatography.

摘要: Chloroquine and de-ethylchloroquine were extracted from plasma blood cells by addition of 80 µl internal standard [2.5 µg/ml 4-(4-dimethylamino-1-methylbutylamino)-7-chloroquinoline], 1 ml 1M-NaOH 6 diethyl ether with shaking for 15 min. After centrifugation at 1000 g 10 min, the layer was evaporated under N2 residue dissolved in mobile phase. HPLC performed on a 5 µm µPorasil column ( 30 cm × 3.9 mm i.d.) dichloromethane/methanol/1M-perchloric acid (100:9:1.2) as phase (1 ml/min) UV detection 343 nm or fluorimetric 380 (excitation 340 nm). Calibration graphs linear 0.025-1 limit 5-10 ng. Within-day day-to-day RSD (n=5) chloroquine 2.3-2.76% 2.83-3.36% de-ethylchloroquine. Recoveries 89.7-91.05%.