Involvement of fatty acid synthase in dengue virus infection

摘要: The mosquito transmitted Dengue virus (DENV) remains a significant public health problem in many tropical and subtropical countries. Increasing evidence has suggested that during the infection process cellular lipids play important roles at several stages of replication cycle. This study sought to characterize changes lipid metabolism gene expression investigated role one enzyme, fatty acid synthase, DENV infection. Transcriptional profiles genes associated with were evaluated by real-time PCR after different cell lines (HepG2 HEK293T/17) DENVs (laboratory adapted low passage). Expression western blotting. A critical protein, synthase was down-regulated through siRNA inhibited orlistat effect on determined flow cytometry, plaque assay, blotting confocal microscopy. results showed alterations transcription seen variously lipogenesis, lipolysis β-oxidation Interference either or had marked effects production, having an EC50 value 10.07 μM 24 h post However, non-structural protein largely unaffected. While drug treatment reduced titer up 3Log10, no observed, suggesting acts virion formation.