The free solution electrophoretic mobility of peptides by a bead modeling methodology.

摘要: A bead modeling methodology, BMM, discussed previously to compute the free solution electrophoretic mobility of peptides [H. Pei, Y. Xin, S.A. Allison, J. Sep. Sci. 31 (2008) 554–564], is generalized avoid approximation orientationally preaveraging hydrodynamic interaction. In general, peptide mobilities computed without are lower by about 2%. The BMM then used study several insect oostatic reported in a variety different buffer systems ranging pH from 2.25 8.1 [V. Solinova, V. Kasicka, D. Koval, Hlavacek, Electrophoresis, 25 (2004) 2299–2308]. With minor adjustment intrinsic pKa0 N-terminal peptide, good agreement between and experiment achieved for models with random secondary structures entire range. Model these appear be relatively insensitive assumed structure.