Chemical Biology Approaches Reveal Conserved Features of a C‐Terminal Processing PDZ Protease

摘要: Several proteases like the high temperature requirement A (HtrA) protein family containing internal or C-terminal PDZ domains play key roles in quality control cell envelope of Gram-negative bacteria. While several HtrA have been extensively characterized, many features processing such as tail-specific protease (Tsp) are still unknown. To fully understand these cellular systems, individual need to be targeted by specific peptides acting activators inhibitors. Here, we describe identification and design potent inhibitors Tsp. Suitable synthetic substrates Tsp were identified served a basis for generation boronic acid-based peptide In addition, proteomic screen E. coli proteins using library was performed identify capable amplifying Tsp's proteolytic activity. The implications findings regulation future mechanistic studies discussed.