Optimization of the process of inverted peptides (PIPEPLUS) to screen PDZ domain ligands

摘要: PDZ domains play crucial roles in cell signaling processes and are therefore attractive targets for the development of therapeutic inhibitors. In many cases, C-terminal peptides physiological binding partners domains. To identify both native ligands potential inhibitors we have screened arrays synthesized by process inverted (PIPE), a variant SPOT synthesis that generates with free C-termini. Here, present new functionalized cellulose membrane as solid support along optimized PIPEPLUS technology. Improved resolution accuracy were shown peptide containing natural non-natural amino acids. These screening possibilities will advance active, selective metabolically stable interactors.