The application of minisatellite variant repeat mapping by PCR (MVR-PCR) in a paternity case showing false exclusion due to STR mutation.
作者： Rieko Uchihi , Yoshinao Katsumata , Alec J. Jeffreys , Toshimichi Yamamoto , Keiji Tamaki
DOI: 10.1520/JFS14976J
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摘要: A boy and a girl with their mother brought paternity suit against an alleged but deceased father. We tested six conventional genetic markers, the AmpliType PM+ DQA1 twelve STR loci children together paternal grandparents. also DNA typed bloodstain found later in father's medical record. Only result at D3S1358 nineplex system excluded father from parentage of boy, whereas all markers were inclusive for girl. Accordingly, we performed sequence analysis to confirm presence exclusion or mutation. The indicated that boy's allele 17 could have originated either 16 18 by single-step mutation associated slippage loci. carried out minisatellite variant repeat mapping PCR (MVR-PCR) D1S8 (MS32) D7S21 (MS31A) mapped haplotypes individuals except MVR-PCR showed has no inconsistency relationship between grandparents, was very effective increasing index (PI) value. conclude there is biological not only
sci-hub.st 参考文章(22)
Keiji Tamaki, Xiu-Lin Huang, Toshimichi Yamamoto, Rieko Uchihi, Hideki Nozawa, Yoshinao Katsumata, Applications of Minisatellite Variant Repeat (MVR) Mapping for Maternal Identification from Remains of an Infant and Placenta Journal of Forensic Sciences. ,vol. 40, pp. 695- 700 ,(1995) , 10.1520/JFS13854J
Keiji Tamaki, Xiu-Lin Huang, Masaki Mizutani, Toshimichi Yamamoto, Ryu Katsumata, Rieko Uchihi, Yoshinao Katsumata, Alec J. Jeffreys, The potential contribution of MVR-PCR to paternity probabilities in a case lacking a mother. Journal of Forensic Sciences. ,vol. 44, pp. 863- 867 ,(1999) , 10.1520/JFS14569J
Toshimichi Yamamoto, Rieko Uchihi, Hideki Nozawa, Xiu-Lin Huang, Yim-Kheng Leong, Miwa Tanaka, Masaki Mizutani, Keiji Tamaki, Yoshinao Katsumata, Allele Distribution at Nine STR Loci—D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820—in the Japanese Population by Multiplex PCR and Capillary Electrophoresis Journal of Forensic Sciences. ,vol. 44, pp. 167- 170 ,(1999) , 10.1520/JFS14426J
Toshimichi Yamamoto, Keiji Tamaki, Toshinori Kojima, Rieko Uchihi, Yoshinao Katsumata, Potential Forensic Applications of Minisatellite Variant Repeat (MVR) Mapping Using the Polymerase Chain Reaction (PCR) at D1S8 Journal of Forensic Sciences. ,vol. 39, pp. 13651J- 750 ,(1994) , 10.1520/JFS13651J
Alec J. Jeffreys, Keiji Tamaki, Annette MacLeod, Darren G. Monckton, David L. Neil, John A.L. Armour, Complex gene conversion events in germline mutation at human minisatellites Nature Genetics. ,vol. 6, pp. 136- 145 ,(1994) , 10.1038/NG0294-136
Hua Li, Laura Schmidt, Ming-Hui Wei, Thomas Hustad, Michael I. Lerman, Berton Zbar, Kalman Tory, Three tetranucleotide polymorphisms for loci: D3S1352; D3S1358; D3S1359 Human Molecular Genetics. ,vol. 2, pp. 1327- 1327 ,(1993) , 10.1093/HMG/2.8.1327
G. Mertens, N. Mommers, H. Heylen, M. Gielis, L. Muylle, A. Vandenberghe, Allele frequencies of nine STR systems in the Flemish population and application in parentage testing. International Journal of Legal Medicine. ,vol. 110, pp. 177- 180 ,(1997) , 10.1007/S004140050062
Alec J. Jeffreys, Annette MacLeod, Keiji Tamaki, David L. Neil, Darren G. Monckton, Minisatellite repeat coding as a digital approach to DNA typing Nature. ,vol. 354, pp. 204- 209 ,(1991) , 10.1038/354204A0
Z. WONG, V. WILSON, I. PATEL, S. POVEY, A. J. JEFFREYS, Characterization of a panel of highly variable minisatellites cloned from human DNA. Annals of Human Genetics. ,vol. 51, pp. 269- 288 ,(1987) , 10.1111/J.1469-1809.1987.TB01062.X
David L. Nell, Alec J. Jeffreys, Digital DNA typing at a second hypervariable locus by minisatellite variant repeat mapping Human Molecular Genetics. ,vol. 2, pp. 1129- 1135 ,(1993) , 10.1093/HMG/2.8.1129