Characterization of a panel of highly variable minisatellites cloned from human DNA.

摘要: Summary Five of the most variable loci detected in human DNA by hybridization with fingerprint probes have been cloned and characterized. Each locus consists a tandem-repetitive minisatellite, repeat units ranging length from 9 to 45 base pairs depending on locus. All these minisatellites act as locus-specific probes, detect extremely Mendelian heterozygosities 90 99%. These five hypervariable loci, together previously-isolated minisatellite designated pΛg3, are dispersed over four autosomes (chromosomes 1, 5, 7 12). Syntenic chromosomes 1 show no detectable pair-wise linkage, thus evidence clustering within genome should provide valuable markers for mapping inherited disease. The very sensitive can be pooled several simultaneously. applications individual identification, paternity testing analysis cell chimaerism discussed, illustrated an forensic specimens two victims who had sexually assaulted murdered. We grateful Professor J. Dausset Dr H. Cann at Human Polymorphism Study Centre, Paris. generous provision samples panel CEPH families, Mary Davis providing JDA hybrids, also following people kindly allowed us use their hybrids. Ellen Solomon. Nigel Spurr. P. Goodfellow, Denise Sheer, John Cowell Ben Carritt. We thank Anabel Kearney did enzyme Lynne West karyotyping. Leicestershire Constabulary permission cite details analysis. A.J.J. is Lister Institute Research Fellow, this work was supported grant Medical Council. subject Patent Applications, commercial enquiries addressed Preventive Medicine, Brockley Hill, Stanmore. Middlesex, H A7 4JD, U.K.