The effect of ecdysterone on nonihistone proteins in salivary gland chromosomes of Sciara coprophila

作者: Reba M. Goodman , Elena Caryn Schmidt , William B. Benjamin

DOI: 10.1016/0045-6039(76)90034-8

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摘要: Synthesis and transport of proteins to the cell nucleus during puff induction was studied in S. coprophila. Changes grain distribution along chromosomes (L-methionine [35S] incorporation into protein) were correlated with puffs induced by ecdysterone vitro; A pattern specific labelling at sites incipient noted within 2 h after addition hormone, i.e. grains on clusters, characteristic for this time point not seen controls (where only non-specific labeling 0-4 h). Characteristic chromosomal appeared between 3-4 ecdysterone. It concluded that ecdysterone-induced formation salivary gland chromosomes, which had been previously synthesized selectively transported from cytoplasm chromosomes.

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