Epigenetic Gene Inactivation Induced by a Cis-acting Methylation Center
作者： Padmaja Mummaneni , Kimberly A. Walker , Peggy L. Bishop , Mitchell S. Turker
关键词:
摘要: Abstract In this report we test the hypothesis that a cis-acting methylation center can induce epigenetic gene inactivation. The element used is an 838-base pair fragment was shown previously to provide de novo signal (Mummaneni, P., Bishop, P. L., and Turker, M. S.(1993) J. Biol. Chem. 268, 552-558). Its normal location approximately 1.3 kilobase pairs upstream of mouse aprt (adenine phosphoribosyltransferase) gene. To determine if could inactivation gene, plasmid construct created in which moved next promoter. Transfection experiments demonstrated on hybrid construct. event with Southern blot analysis correlate hypermethylation be reversible by treatment 2-deoxy-5′-azacytidine, demethylating agent. Interestingly, induced required truncation results demonstrate DNA center.
elsevier.com
sci-hub.se 参考文章(24)
P Vertino, M Makos, S B Baylin, A de Bustros, R W Yen, J J Wu, B D Nelkin, Abnormal patterns of DNA methylation in human neoplasia: Potential consequences for tumor progression Cancer Cell. ,vol. 3, pp. 383- 390 ,(1991)
A. Hasse, W.A. Schulz, Enhancement of reporter gene de novo methylation by DNA fragments from the alpha-fetoprotein control region. Journal of Biological Chemistry. ,vol. 269, pp. 1821- 1826 ,(1994) , 10.1016/S0021-9258(17)42100-4
P. Mummaneni, P.L. Bishop, M.S. Turker, A cis-acting element accounts for a conserved methylation pattern upstream of the mouse adenine phosphoribosyltransferase gene. Journal of Biological Chemistry. ,vol. 268, pp. 552- 558 ,(1993) , 10.1016/S0021-9258(18)54187-9
M S Turker, K Swisshelm, A C Smith, G M Martin, A partial methylation profile for a CpG site is stably maintained in mammalian tissues and cultured cell lines. Journal of Biological Chemistry. ,vol. 264, pp. 11632- 11636 ,(1989) , 10.1016/S0021-9258(18)80110-7
J Toguchida, T Sakai, N Ohtani, D W Yandell, T P Dryja, J M Rapaport, Allele-specific hypermethylation of the retinoblastoma tumor-suppressor gene. American Journal of Human Genetics. ,vol. 48, pp. 880- 888 ,(1991)
J. Boyes, A. Bird, Repression of genes by DNA methylation depends on CpG density and promoter strength: evidence for involvement of a methyl-CpG binding protein. The EMBO Journal. ,vol. 11, pp. 327- 333 ,(1992) , 10.1002/J.1460-2075.1992.TB05055.X
M Szyf, G Tanigawa, P L McCarthy, A DNA signal from the Thy-1 gene defines de novo methylation patterns in embryonic stem cells. Molecular and Cellular Biology. ,vol. 10, pp. 4396- 4400 ,(1990) , 10.1128/MCB.10.8.4396
Mitchell S. Turker, Annette C. Smith, George M. Martin, High frequency ?switching? at the adenine phosphoribosyltransferase locus in multipotent mouse teratocarcinoma stem cells Somatic Cell and Molecular Genetics. ,vol. 10, pp. 55- 69 ,(1984) , 10.1007/BF01534473
Michael Brandeis, Dale Frank, Iiana Keshet, Zahava Siegfried, Monica Mendelsohn, Adriana Names, Violet Temper, Aharon Razin, Howard Cedar, Sp1 elements protect a CpG island from de novo methylation Nature. ,vol. 371, pp. 435- 438 ,(1994) , 10.1038/371435A0
D Macleod, J Charlton, J Mullins, A P Bird, Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island. Genes & Development. ,vol. 8, pp. 2282- 2292 ,(1994) , 10.1101/GAD.8.19.2282