Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri.
作者： Charlotte Philippe , Arnout F. Gregoir , Eli S. J. Thoré , Gudrun De Boeck , Luc Brendonck
DOI: 10.3791/57308
关键词:
摘要: The killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability acute chronic ecotoxicity testing has been demonstrated. Overall, sensitivity species to toxic compounds range with, or higher than, that other species. This work describes protocols for acute, chronic, multigenerational bioassays single combined stressor effects on N. furzeri. Due short maturation time life-cycle, this vertebrate allows study endpoints such as fecundity within four months. Transgenerational full life-cycle exposure trials can be performed little 8 Since produces eggs are drought-resistant remain viable years, on-site culture not needed but individuals recruited when required. designed measure life-history traits (mortality, growth, fecundity, weight) critical thermal maximum.
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