Variations on Fibrinogen-Erythrocyte Interactions during Cell Aging

摘要: Erythrocyte hyperaggregation, a cardiovascular risk factor, is considered to be caused by an increase in plasma adhesion proteins, particularly fibrinogen. We have recently reported specific binding between fibrinogen and erythrocyte integrin receptor with β3 or β3-like subunit. In this study we evaluate the influence of aging on binding. By atomic force microscopy-based spectroscopy measurements found that increasing age, there decrease decreasing frequency its occurrence but not force. This observation reinforced zeta-potential fluorescence measurements. conclude upon number molecules bound each cell decreases significantly, due progressive impairment fibrinogen-erythrocyte interaction. Knowing younger erythrocytes bind more fibrinogen, could presume population main contributor diseases associated increased content blood, which disturb blood flow. Our data also show sialic acids exposed membrane contribute for interaction possibly facilitating receptor.