Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm.

摘要: Abstract The objective was to determine the optimal concentration of heparin for sperm capacitation, as well in vitro fertilization using flow cytometrically-sorted from individual bulls. A total 5327 bovine oocytes and four bulls were examined. Oocytes slaughterhouse ovaries matured TCM199 22–24 h. Flow unsorted control same cryopreserved. For each bulls, inseminated a three-by-three factorial design plus one group (three concentrations: 0, 2, 10 μg/ml three 0.5×106, 1.5×106, 4.5×106 ml−1; 1.5×106 ml−1 used control). Presumptive zygotes cultured chemically defined media, CDM-1 CDM-2 52–54 h 96 h, respectively. Samples about 10 treatment groups per replicate fixed at 18–20 h after insemination pronuclei formation polyspermy. Increased polyspermy resulted concentrations increased (P