Label-free measurement of cell-electrode cleft gap distance with high spatial resolution surface plasmon microscopy.

摘要: Understanding the interface between cells or tissues and artificial materials is of critical importance for a broad range areas. For example, in neurotechnology, interfaces neurons external devices create link technical nervous systems by stimulating recording from neural tissue. Here, more effective required to enhance electrical characteristics neuronal recordings stimulations. Up now, lack systematic characterization cell–electrode interaction turns out be major bottleneck. In this work, we employed recently developed surface plasmon microscope (SPM) monitor real-time cell–metal measure situ gap distance cleft with spatial resolution reaching optical diffraction limit. The SPM allowed determination human embryonic kidney 293 cultured on gold surfaces coated various peptides proteins without any labeling. This method can dramatical...