Structure and sequence evolution of three legume chloroplast DNAs

摘要: Chloroplast DNAs from soybean (Glycine max), common bean (Phaseolus vulgaris) and mung (Vigna radiata) have been compared in overall structure nucleotide sequence homologies. Detailed restriction maps demonstrate that the genomes possess classical large chloroplast DNA inverted repeat, encoding ribosomal RNA genes, as found previously (Palmer Thompson 1981 a). Heterologous filter hybridizations indicate essentially complete colinearity between DNAs. Although linear order of elements is also conserved DNAs, two regions deletions/additions, each totaling almost 5 kilobase pairs size, identified at ends single copy region. Alignment comparison has allowed calculation divergence values for three Mung differ by an average 10–13% sequence, while are significantly more closely related, differing only 5–6% base sequence. Base substitutions distributed non-randomly these DNAs; relatively deletion/addition diverged