Induction of Vascular Endothelial Growth Factor Gene Expression by Interleukin-1 β in Rat Aortic Smooth Muscle Cells (∗)

摘要: Vascular endothelial growth factor (VEGF) is a potent and specific mitogen for vascular cells promotes neovascularization in vivo. To determine whether interleukin-1β (IL-1β), which present atherosclerotic lesions, induces VEGF gene expression smooth muscle cells, we performed RNA blot analysis on rat aortic (RASMC) with cDNA probe. IL-1β increased mRNA levels RASMC time- dose-dependent manner. As little as 0.1 ng/ml by 2-fold 10 4-fold. We also measured the half-life of nuclear run-on experiments before after addition to see if stabilizing or increasing its rate transcription. The normal, 2-h was lengthened 3.2 h (60%) IL-1β, transcription 2.1-fold. In immunoblot an antibody VEGF, found that protein 3.3-fold. Together these data indicate cells. This IL-1β-induced may accelerate progression lesions promoting development new blood vessels.