Activation of phosphatidylinositol-3' kinase by Src-family kinase SH3 binding to the p85 subunit
作者： C. Pleiman , W. Hertz , J. Cambier
关键词:
摘要: Engagement of antigen receptor complexes induces rapid activation Src-family kinases and association with phosphatidylinositol-3' kinase (PI-3 kinase). Here it was found that the Src homology 3 (SH3) domain Lyn Fyn bound to a proline-rich region (residues 84 99) within 85-kilodalton subunit (p85) PI-3 kinase. The binding SH3 purified led five- sevenfold increase in specific activity Ligand-induced stimulation activated kinase, this blocked by peptide containing residues 99 p85. These data demonstrate mechanism for show domains target sequences can regulate enzymatic activity.
sciencemag.org
harvard.edu
unirioja.es参考文章(50)
C. W. Turck, A. L. Defranco, M. R. Gold, V. W.-F. Chan, Membrane Ig cross-linking regulates phosphatidylinositol 3-kinase in B lymphocytes. Journal of Immunology. ,vol. 148, pp. 2012- 2022 ,(1992)
H Hayashi, S Kamohara, Y Nishioka, F Kanai, N Miyake, Y Fukui, F Shibasaki, T Takenawa, Y Ebina, Insulin treatment stimulates the tyrosine phosphorylation of the alpha-type 85-kDa subunit of phosphatidylinositol 3-kinase in vivo. Journal of Biological Chemistry. ,vol. 267, pp. 22575- 22580 ,(1992) , 10.1016/S0021-9258(18)41711-5
G Endemann, K Yonezawa, R A Roth, Phosphatidylinositol Kinase or an Associated Protein Is a Substrate for the Insulin Receptor Tyrosine Kinase Journal of Biological Chemistry. ,vol. 265, pp. 396- 400 ,(1990) , 10.1016/S0021-9258(19)40243-3
Y.R. Hadari, E Tzahar, O Nadiv, P Rothenberg, C.T. Roberts, D LeRoith, Y Yarden, Y Zick, Insulin and insulinomimetic agents induce activation of phosphatidylinositol 3'-kinase upon its association with pp185 (IRS-1) in intact rat livers. Journal of Biological Chemistry. ,vol. 267, pp. 17483- 17486 ,(1992) , 10.1016/S0021-9258(19)37065-6
J.M. Backer, M.G. Myers, S.E. Shoelson, D.J. Chin, X.J. Sun, M. Miralpeix, P. Hu, B. Margolis, E.Y. Skolnik, J. Schlessinger, Phosphatidylinositol 3'-kinase is activated by association with IRS-1 during insulin stimulation. The EMBO Journal. ,vol. 11, pp. 3469- 3479 ,(1992) , 10.1002/J.1460-2075.1992.TB05426.X
D S Wages, J Keefer, T B Rall, M J Weber, Mutations in the SH3 domain of the src oncogene which decrease association of phosphatidylinositol 3'-kinase activity with pp60v-src and alter cellular morphology. Journal of Virology. ,vol. 66, pp. 1866- 1874 ,(1992) , 10.1128/JVI.66.4.1866-1874.1992
S.G. Ward, K Reif, S Ley, M.J. Fry, M.D. Waterfield, D.A. Cantrell, Regulation of phosphoinositide kinases in T cells. Evidence that phosphatidylinositol 3-kinase is not a substrate for T cell antigen receptor-regulated tyrosine kinases. Journal of Biological Chemistry. ,vol. 267, pp. 23862- 23869 ,(1992) , 10.1016/S0021-9258(18)35916-7
A. Kashishian, A. Kazlauskas, J.A. Cooper, Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. The EMBO Journal. ,vol. 11, pp. 1373- 1382 ,(1992) , 10.1002/J.1460-2075.1992.TB05182.X
L Varticovski, G Q Daley, P Jackson, D Baltimore, L C Cantley, Activation of phosphatidylinositol 3-kinase in cells expressing abl oncogene variants. Molecular and Cellular Biology. ,vol. 11, pp. 1107- 1113 ,(1991) , 10.1128/MCB.11.2.1107
P Hu, B Margolis, E Y Skolnik, R Lammers, A Ullrich, J Schlessinger, Interaction of phosphatidylinositol 3-kinase-associated p85 with epidermal growth factor and platelet-derived growth factor receptors. Molecular and Cellular Biology. ,vol. 12, pp. 981- 990 ,(1992) , 10.1128/MCB.12.3.981