Characterization of epididymal sperm motion and its correlation with stages of target cells in rats given α-chlorohydrin, cyclophosphamide or nitrazepam

作者: Masako KANETO , Susumu KANAMORI , Katsumi HARA , Kurajiro KISHI

DOI: 10.2131/JTS.24.3_187

关键词:

摘要: Epididymal sperm motion in rats was characterized by computer-aided analysis (CASA) with its correlation to testicular lesions the 2-week treatment study, using three compounds which are known affect different stages of germ cells. Mature male were treated daily for 2 weeks alpha-chlorohydrin (alpha-CH, 5 mg/kg), cyclophosphamide (CP, 20 mg/kg) or nitrazepam (NZ, 20, 40, 60 mg/kg). Changes detected only alpha-CH and 60-mg/kg NZ-treated groups. Of parameters, velocity amplitude lateral head displacement (ALH) concomitantly reduced these two groups good correlation. With respect distribution values however, shifted down within a small range high percentages motile sperm, while NZ distributed them over wide low sperm. CP showed no histopathological changes advanced cells, though it decrease number early affected round elongating spermatids (approximately step 14) at doses 40 mg/kg, also more 19) dose mg/kg. did not histopathology. These findings indicate that as result elongated direct effects on epididymal spermatozoa. The present study indicates addition percentage ALH can be useful detect caused actions alpha-CH, each compound distinct pattern parameters.

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