Lectin binding to cutaneous malignant melanoma: HPA is associated with metastasis formation

摘要: Changes in protein glycosylation of tumour cells, as detected by lectin histochemistry, have been associated with metastasis formation several human malignancies. This study analysed the association between binding and cutaneous malignant melanoma. In a 10-year retrospective study, sections 100 primary melanomas were histochemically stained for following 5 lectins: HPA, SNA-I, MAA, WGA PHA-L, differing their carbohydrate specificity. Since differences results HPA depending on methodology reported, an indirect biotinylated method employed HPA. Kaplan-Meier analysis time to first revealed positive correlation both methods, (P< 0.0001) being superior 'indirect' (P = 0.0006). Cox regression demonstrated that even after adjustment stage, positivity is independent predictor metastasis. The present indicate N -acetyl-galactosamine/-glucosamine residues, recognized are linked contrast, beta1-6 branched oligosaccharides or sialic acid which correlated other malignancies, no functional importance Thus, proved be useful prognostic marker metastatic phenotype