The role of extracellular factors in human metastatic chordoma cell growth in vitro

摘要: Study design Human metastatic chordoma cells were isolated, and initial in vitro characterization was performed. Biochemical physiologic changes observed response to pH, oxygen, glucose. Objective The extracellular microenvironment directly affects cell phenotype vitro. Summary of background data Chordomas are primary bone tumors that usually occur the spine or skull. arise from embryonic notochordal remnants along axial skeleton, most commonly sacrum, followed by base skull mobile spine. Due a high degree resistance radiation chemotherapy, chordomas eventually cause death direct growth metastasizing other organs. Methods Extracellular glucose levels culture medium controlled, assessed using MTT staining, SDS-PAGE, Western blotting, tandem mass spectrometry, TUNEL, immunofluorescence, flow cytometry. Results In this study, we present new line established tissue novel characterizing some aspects different conditions Chordoma biologic markers expressed line. Alkaline pH dramatically increased intracellular protein tyrosine phosphorylation, metabolic activity, albumin accumulation cells, while acidic caused apoptosis. Conclusion level proliferation, apoptosis, as well overall expression profile, strongly depended on media oxygen/glucose levels. Chordoma's preferred rather alkaline, with an optimum at 8.5, apoptotic induced pH. We found bovine serum accumulated incubation media, highest alkaline