Establishment and characterization of a novel chordoma cell line: CH22.

摘要: Chordoma is a rare primary malignant bone tumor and there exist only few established human chordoma cell lines. The scarcity of robust lines has limited the ability to study this tumor. In report, we describe establishment novel line characterize its in vitro vivo behaviors. tissue was isolated from patient with recurrent sacrum. After 6 months culture, line, referred here as CH22, established. Microscopic analysis two-dimensional culture confirmed that CH22 cells exhibited typical vacuolated cytoplasm similar well-established U-CH1. Electron microscopy showed cohesive numerous surface filopodia, pockets glycogen aggregates intermediate tonofilaments cytoplasm. Three-dimensional revealed could grow form clusters by day 8. MTT assays demonstrated that, compared sensitive osteosarcoma lines, were relatively resistant conventional chemotherapeutic drugs. Western blotting immunofluorescence expressed brachyury, vimentin, cytokeratin. Finally, histological xenograft tissues appearance physaliphorous positive staining cytokeratin, S100. By CT MRI, imaging xenografts appearances seen chordomas. These findings suggest tumorigenecity SCID nude mice may serve an important model for studying biology development new therapeutic modalities.