4‐Hydroperoxyclophosphamide: a model for eliminating residual human tumour cells and T‐lymphocytes from the bone marrow graft

摘要: Summary. Autologous bone marrow transplantation in acute leukaemia carries the risk of relapse from reinfusion tumour cells present collected remission and cryopreserved. An effective method for clearing required a successful outcome. In animal model 4-hydroperoxycyclophosphamide (4-HC) has proved to be eliminating an autologous graft. studies, vitro effect short-and long-term cell incubation with 4-HC on haemopoietic stem was investigated determine maximum concentration that can used without destroying capacity complete haematological recovery as judged by residual CFUc content. However, loss may not necessarily parallel survival pluripotential cells. also shown against peripheral T-lymphocytes. Its possible therapeutic use preventing or ameliorating graft-versus-host disease allogeneic grafts preincubation prior is discussed.