Ultrasensitive and absolute quantification of the phosphoinositide 3-kinase/Akt signal transduction pathway by mass spectrometry

摘要: The phosphoinositide 3-kinase (PI3K)/Akt pathway controls a vast array of normal physiological processes and is frequently aberrantly activated in cancer, thus identifying PI3K/Akt-signaling components as promising drug targets oncology. However, implementation rational cancer therapies for this needs robust simple tools to stratify patients according PI3K activation validate measure the impact targeted inhibition on primary tissues. Herein we present technique quantification based mass spectrometric measurement PI3K-dependent protein kinase activity cell lysates. concept application MS exploit enzymatic amplify signal enzyme under study analogous PCR used nucleic acid sequences. We show that approach allows quantitative analysis cell-signaling with high sensitivity, precision quantification, specificity. Due its special analytical capabilities potential multiplexing, could contribute significantly studies development personalized therapies.