Remotely plucked hair genotyping: a reliable and non-invasive method for censusing pine marten (Martes martes, L. 1758) populations
作者： Jacinta Mullins , Mark J. Statham , Tom Roche , Peter D. Turner , Catherine O’Reilly
DOI: 10.1007/S10344-009-0332-X
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摘要: We investigated the feasibility of using genetic techniques to census pine marten (Martes martes) populations by genotyping non-invasively collected samples (plucked hair and scats), with particular reference genetically depauperate Irish population. Novel real-time polymerase chain reaction methods were developed for species sex identification, targeting short DNA sequences. Background variation at 17 microsatellite loci was very low in population, an average 2.29 alleles per locus expected heterozygosity 0.35. Despite such polymorphism, a panel eight sibling probability identity 0.011 reliably identified individual their gender, as determined genotypes live trapped individuals. With high nuclear amplification success rates (93.8%) error (1.8%), plucked hairs may represent more reliable cost-effective source than scats monitoring this elusive carnivore, similar taxa sympatric stone Martes foina.
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sci-hub.se 参考文章(61)
Johnny Birks, John Messenger, Tony Braithwaite, Angus Davison, Rachael Brookes, Chris Strachan, Are Scat Surveys a Reliable Method for Assessing Distribution and Population Status of Pine Martens? Martens and Fishers (Martes) in Human-Altered Environments. pp. 235- 252 ,(2005) , 10.1007/0-387-22691-5_12
Garth Mowat, David Paetkau, Estimating marten Martes americana population size using hair capture and genetic tagging Wildlife Biology. ,vol. 8, pp. 201- 209 ,(2002) , 10.2981/WLB.2002.034
D Yalden, S Harris, Mammals of the British Isles: Handbook ,(2008)
CHUAN-CHIN HUANG, YU-CHENG HSU, LING-LING LEE, SHOU-HSIEN LI, Isolation and characterization of tetramicrosatellite DNA markers in the Eurasian otter (Lutra lutra) Molecular Ecology Notes. ,vol. 5, pp. 314- 316 ,(2005) , 10.1111/J.1471-8286.2005.00912.X
ALAIN C. FRANTZ, MICHEL SCHAUL, LISA C. POPE, FRED FACK, LAURENT SCHLEY, CLAUDE P. MULLER, TIMOTHY J. ROPER, Estimating population size by genotyping remotely plucked hair: the Eurasian badger Journal of Applied Ecology. ,vol. 41, pp. 985- 995 ,(2004) , 10.1111/J.0021-8901.2004.00951.X
Daniel J. Harrison, Angela K. Fuller, Gilbert Proulx, Martens and Fishers (Martes) in human-altered environments : an international perspective Springer. ,(2004)
Huw I. Griffiths, Angus Davison, Johnny D. S. Birks, Rachael C. Brookes, John E. Messenger, Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Molecular Ecology. ,vol. 10, pp. 2479- 2488 ,(2002) , 10.1046/J.1365-294X.2001.01381.X
Daniel Dieringer, Christian Schlötterer, microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets Molecular Ecology Notes. ,vol. 3, pp. 167- 169 ,(2003) , 10.1046/J.1471-8286.2003.00351.X
A. B. LYNCH, M. J. F. BROWN, Molecular sexing of pine marten (Martes martes): how many replicates? Molecular Ecology Notes. ,vol. 6, pp. 631- 633 ,(2006) , 10.1111/J.1471-8286.2006.01386.X