HNF1A-AS1 Regulates Cell Migration, Invasion and Glycolysis via Modulating miR-124/MYO6 in Colorectal Cancer Cells
作者： Xiong Guo , Yang Zhang , Ling Liu , Weiming Yang , Qi Zhang
DOI: 10.2147/OTT.S231249
关键词:
摘要: Background Accumulating evidence determined that lncRNAs play multiple roles in cell progression colorectal cancer (CRC). Long noncoding RNA (lncRNA) hepatocyte nuclear factor 1 homeobox A (HNF1A)-antisense (AS1) has been identified to affect growth and disease diagnosis various cancers, including CRC. However, the underlying regulatory mechanism of HNF1A-AS1 glycolysis not fully explored Materials methods The expression HNF1A-AS1, microRNA-124 (miR-124) Myosins class VI (MYO6) was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). analysis glucose consumption, lactate production hexokinase 2 (HK2) protein level used assess cells. HK2 MYO6 measured with Western blot. Cell migration invasion were evaluated transwell assay. relationship among miR-124 via luciferase reporter immunoprecipitation (RIP) Results In this study, we found upregulated CRC tissues lines. Functional experiments reduction or promotion inhibited as well What' more, assay manifested a target mRNA Additionally, showed effects si-HNF1A-AS1 on cells impaired by anti-miR-124 high rescued upregulating MYO6. regulated targeting Conclusion first migration, modulating miR-124/MYO6
scilit.net参考文章(52)
Lance A. Liotta, Tumor Invasion and Metastases—Role of the Extracellular Matrix: Rhoads Memorial Award Lecture Cancer Research. ,vol. 46, pp. 1- 7 ,(1986)
Ying Wu, Hongbing Liu, Xuefei Shi, Yanwen Yao, Wen Yang, Yong Song, The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma. Oncotarget. ,vol. 6, pp. 9160- 9172 ,(2015) , 10.18632/ONCOTARGET.3247
Anne J. Ridley, Rho GTPases and cell migration. Journal of Cell Science. ,vol. 114, pp. 2713- 2722 ,(2001) , 10.1242/JCS.114.15.2713
Zhengkun Li, Xu Li, Shouzhen Wu, Mei Xue, Wei Chen, Long non-coding RNA UCA1 promotes glycolysis by upregulating hexokinase 2 through the mTOR-STAT3/microRNA143 pathway. Cancer Science. ,vol. 105, pp. 951- 955 ,(2014) , 10.1111/CAS.12461
Raul Baena, Pedro Salinas, Diet and colorectal cancer. Maturitas. ,vol. 80, pp. 258- 264 ,(2015) , 10.1016/J.MATURITAS.2014.12.017
Masatoshi Kitagawa, Kyoko Kitagawa, Yojiro Kotake, Hiroyuki Niida, Tatsuya Ohhata, Cell cycle regulation by long non-coding RNAs Cellular and Molecular Life Sciences. ,vol. 70, pp. 4785- 4794 ,(2013) , 10.1007/S00018-013-1423-0
Guodong Yang, Xiaozhao Lu, Lijun Yuan, LncRNA: a link between RNA and cancer. Biochimica et Biophysica Acta. ,vol. 1839, pp. 1097- 1109 ,(2014) , 10.1016/J.BBAGRM.2014.08.012
Yang Zhang, Xiao-Ou Zhang, Tian Chen, Jian-Feng Xiang, Qing-Fei Yin, Yu-Hang Xing, Shanshan Zhu, Li Yang, Ling-Ling Chen, Circular Intronic Long Noncoding RNAs Molecular Cell. ,vol. 51, pp. 792- 806 ,(2013) , 10.1016/J.MOLCEL.2013.08.017
Gabriel Eades, Long non-coding RNAs in stem cells and cancer World journal of clinical oncology. ,vol. 5, pp. 134- 141 ,(2014) , 10.5306/WJCO.V5.I2.134
Joachim Silber, Daniel A Lim, Claudia Petritsch, Anders I Persson, Alika K Maunakea, Mamie Yu, Scott R Vandenberg, David G Ginzinger, C David James, Joseph F Costello, Gabriele Bergers, William A Weiss, Arturo Alvarez-Buylla, J Graeme Hodgson, miR-124 and miR-137 inhibit proliferation of glioblastoma multiforme cells and induce differentiation of brain tumor stem cells. BMC Medicine. ,vol. 6, pp. 14- 14 ,(2008) , 10.1186/1741-7015-6-14