Silica nanoparticles induce reversible damage of spermatogenic cells via RIPK1 signal pathways in C57 mice

摘要: The reproductive toxicity of silica nanoparticles (SiNPs) is well known, but the underlying mechanism still not clear. To investigate toxic SiNPs on spermatogenic cells, 60 C57 male mice were randomly and equally divided into three groups (the control group, saline group) with two observed time points (45 days 75 days). in group administered 2 mg/kg diluted normal saline, given equivoluminal by tracheal perfusion every 3 for 45 (in total 15 times). bred without treatment. In each a half number sacrificed 45th day after first dose, remaining 75th day. results showed that increased malformation sperms decreased motility concentration epididymis dose. induced oxidative stress testis led to apoptosis necroptosis cells. Furthermore, expression Fas/FasL/RIPK1/FADD/caspase-8/caspase-3 RIPK3/MLKL However, compared index Fas/FasL/RIPK1/FADD/caspase-8/caspase-3/RIPK3/MLKL no significant changes These data suggested could induce cells activating RIPK1 pathway resulting from mice. SiNPs-induced damage recovered which reversible.