Ethanol-induced cell death in cultured rat astroglia

摘要: Because of the important role glial cells in brain maturation and reports on their delayed proliferation following ethanol exposure, it was considered interest to investigate mechanism action these cells. Biochemical parameters related apoptotic necrotic processes astroglial exposed for 1 week 50 100 mM were examined. Ethanol increased intracellular calcium levels without changing transglutaminase activity nitrite levels. Moreover, DNA fragmentation noted with flow cytometry random oligonucleotide primed synthesis assay but neither agar gel electrophoresis nor UV microscopy cell nuclei. The patterns obtained different from seen programmed death. Additionally, immunocytochemical analysis showed greater fragility astrocytes than oligodendrocytes ethanol. These results support hypothesis that vitro die due not mechanisms.